IVF is a process by which eggs are recovered from the ovary , fertilized in the laboratory and transferred into the uterus 3 - 5 days later as cleaving embryos or blastocysts.

Ovulation induction was performed to stimulate the ovaries to produce several eggs for IVF in order to increase the chance of conception. Once more than three follicles have ripened , the female partner will be given an injection of hCG which will trigger the final maturation process of the eggs. The egg recovery will be performed 36 hours after hCG administration and on that day the husband will be required to produce semen samples. The eggs are recovered from the follicles using ultrasound- guided technique. The oocytes are carefully identified and washed before being placed in incubator. After a period of time , usually 3 - 4 hours, they are inseminated with the specially prepared sperm. The oocytes and sperm are cultured together and inspected on the following day to see if fertilization has occurred. The first signs of fertilization , are shown by the presence of two small dots within the egg , the pronuclei. If this has occurred , the zygote ( a fertilized egg ) may then divided into two , four or more - cell embryo.

When the eggs have fertilized and cleaved , no more than three embryos will be transferred into the women on day 3 or day 5 after egg collection. Husbands are encouraged to accompany their wives during this special time , but only they wish to do so. The couple will be shown the embryos before the transfer takes place and the embryos' picture will also be given. If there are more than three good quality embryos , the extra embryos can be frozen for future attempts.

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